Isotopes of one chemical element differ in their masses, but are naturally present in constant ratios of abundance in gemstones. The performance of isotopic analyses using simultaneous acquisition of GemTOF, is expected to be better than that using LA-ICP-Q-MS. The unique natural formation history of gemstones and pearls may lead to slight differences in isotopic ratios in samples. This uniqueness could help to answer questions of carbon source for diamonds or separate synthetic from natural gemstones. Further research is also required with regards to isotopic analysis and origin determination of gemstones such as ruby, sapphire and emeralds.